1.Download python3.4 and biopython1.65+ 
2.Download standalone BLAST from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/ and install it
3.Copy blastn.exe and makeblastdb.exe into the same file directory as Marker Finder.
4.Run the Marker Finder.py

The program may have bugs, if you find one, please contact with yinshiheiyu@163.com. Thanks!